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28 This tactic involves the carboxyterminal nonrepetitive areas current in all MA silks as well as codon-optimized repetitive regions. utilizing a seamless cloning procedure, a controlled mixture of engineered modules and also of authentic gene fragments with no era of undesirable gaps or artifacts Within the coding sequence is feasible.65 Employing this method, it absolutely was probable to considerably boost the expression yields.sixty six The modular method even further enabled qq808 the creation of Unique engineered proteins with tailor-built Attributes for experimental Investigation.
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In distinction to your Flag silk gene, each analyzed MA silk genes deficiency introns consisting in place of unusually significant exons (>nine,000 bp of coding sequence). the massive exons is likely to be a result of gene duplication processes in the course of evolution.
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